HUMAN SYSTEMS QUESTION 1986:               L. PETERSON/AP BIOLOGY

	Beginning at the presynaptic membrane of the neuromuscular junction, describe
	the physical and biochemical events involved in the contraction of a skeletal
	muscle fiber. Include the structure of the fiber in your discussion.

STANDARDS: Presynaptic (must be a motor neuron) 1 - Nature of impulse +++ --- 1 - Increase of permeability of Ca 2+ 1 - Movement of vesicles 1 - Fusion of vesicles with membrane 1 - Transmitter release 1 - Acetylcholine 1 - Diffusion of transmitter (20 nm) 1 - Cholinesterase 1 - Motor end plate or terminal bouton Postsynaptic (muscle be a muscle fiber) A. Transmitter Effects 1 - Specific receptor sites 1 - Causes depolarization/action potential 1 - Action potential sweeps fiber membrane/T. tubules 1 - Release of Ca 2+ from Sarcoplasmic reticulum (T. cisternae) B. Calcium Effects 1 - Calcium floods sarcomere 1 - Calcium binds to troponin 1 - Bound troponin displaces tropomyosin 1 - Calcium returned by active transport (Ca2+ pump) C. Actinomyosin Complex 1 - Binding sites actin exposed by tropomyosin displacement 1 - Myosin cross bridges bind actin 1 - ATP used 1 - Power stroke, recovery flip 1 - ATP needed to separate actin-myosin 1 - Rigor Mortis D. Other 1 - Sliding Filament Theory 1 - Diagram/explanation contracted muscle 1 - Muscle twitch, tetany, fatigue 1 - Lactic acid, O2 debt 1 - Creatine phosphate--ATP Muscle Fiber Structure 1 - Definition 1 - Characteristics 2 - Striations, banding multi-nucleated, many mitochondria 1 - Myofibrils (as sub-units) 1 - Sarcomere (as unit of myofibril) 1 - Sarcoplasmic reticulum (cell membrane, etc.) (terminal cisternae) 1 - T. tubules (cell membrane connection, etc.) 1 - Banding Pattern (explain or diagram) 1 - Actin/myosin (protein or thin, thick, etc.) 1 - Actin a globular protein 1 - Myosin a linear protein w/cross bridges 1 - Troponin/tropomyosin associated with actin