CELLULAR RESPIRATION QUESTION 1989: L. PETERSON/AP BIOLOGY Explain what occurs during the Krebs (citric acid) cycle and electron transportn by describing the following: a. The location of the Krebs cycle and electron transport chain in the mitochondria b. The cyclic nature of the reactions in the Krebs cycle c. The production of ATP and reduced coenzymes during the cycle d. The chemiosmotic production of ATP during electron tranpsort
STANDARDS: 3 points Maximum for each of the four sections 1 point for any of the following: LOCATION ___Description of internal structure (compartmentalization) of mitochondrion ___Krebs in matrix (inner or M compartment) ___Krebs' enzymes mostly dissolved in matrix ___ETS in cristae (inner membrane) ___ETS components are embedded in the inner membrane 5 pts MAX 3 CYCLIC NATURE OF KREBS ___Acetyl CoA (C2) starts Krebs ___C2 joins with OAA (C4) to form citric acid (C6) ___2 CO2 removed during Krebs ___OAA is recycled or overall cycle concept (diagram OK) ___Position of Krebs in Aerobic Respiration (2 cycles/glucose, uses products of glycolysis) 5 pts MAX 3 PRODUCTION OF ATP AND REDUCED COENZYMES ___ 1 ATP/cycle ___NADH and/or FADH2 formed (or NADH2, NADH + H+, NAD red) ___Amount of NADH (3) and/or FADH2 (1) per cycle ___ATP formed from released energy; substrate level phosphorylation ___ATP specific reaction: Succinyl CoA -> Succinic Acid; GTP --> GDP ADT -> ATP ___NADH or FADH2 formed by H or e- 6 pts MAX 3 CHEMIOSMOTIC PRODUCTION OF ATP ___Electron transfer (redox) through carriers; O2 final acceptor ___Gradient drives ATP formation or battery (electrochemical, charge separation, etc.) ___Protons pumped to inter-membrane space; proton carriers alternate w/electron carriers; charge separation; gradient established; lowers pH in inter-membrane space; + between membranes; - matrix ___Inner membrane impermeable to H+ except for and/or proton channel (ATP synthetase; ATPase) is permeable ___ATP synthetase structure F0 + F1 ___Specifics of # of ATPs formed (ETS = 32 ATP; 2 ATP/FADH2; 3ATP/NADH) 6 pts MAX 3